Cell docking inside microwells within reversibly sealed microfluidic channels for fabricating multiphenotype cell arrays

We present a soft lithographic method to fabricate multiphenotype cell arrays by capturing cells within an array of reversibly sealed microfluidic channels. The technique uses reversible sealing of elastomeric polydimethylsiloxane (PDMS) molds on surfaces to sequentially deliver various fluids or cells onto specific locations on a substrate. Microwells on the substrate were used to capture and immobilize cells within low shear stress regions inside channels. By using an array of channels it was possible to deposit multiple cell types, such as hepatocytes, fibroblasts, and embryonic stem cells, on the substrates. Upon formation of the cell arrays on the substrate, the PDMS mold could be removed, generating a multiphenotype array of cells. In addition, the orthogonal alignment and subsequent attachment of a secondary array of channels on the patterned substrates could be used to deliver fluids to the patterned cells. The ability to position many cell types on particular regions within a two dimensional substrate could potentially lead to improved high-throughput methods applicable to drug screening and tissue engineering.     

Oxidations of 4- and 3-Aminopyridinepentaammineruthenium(ii) Complexes by Ethylenediaminetetraacetatocobaltate(III)

Redox reactions of Co(edta)^? with Ru(NH₃)₅L²⁺ (L = 3- and 4-aminopyridine (AmPy)) were found to follow an outer-sphere electron transfer mechanism. The specific rate constants are (3.26 ± 0.03) × 10² and (3.07 ± 0.04) × 10₃ M^?1S^?1, for L = 3- and 4-AmPy, respectively, at μ, = 0.10 M LiClO₄, pH = 8.0 (tris) and T = 25 °C. The rate constants of oxidations for a series of Ru(NH₃)₅L²⁺ complexes are higher than those of the corresponding Fe(CN)₅L_3- complexes by factors of 4 to 15 even after corrections for differences in reduction potentials and in charges of the complexes. Nonadiabaticity in the reactions of Fe(CN)₅L3 complexes may account for the difference in the relative reactivities.     

Spectrophotometric Determination of Zirconium in Monazite Sand

A sensitive spectrophotometric method was developed for the determination of zirconium in monazite sand. The zirconium ion was previously separated from the other interfering elements by a 0.5 M thenoyltrifluoroacetone (TTA)-xylene extraction, then determined with Arsenazo III in 9 M HCl medium. Zirconium reacted with Arsenazo III to form an emeraled green coloured complex having maximum absorbance at 665 nm in 9 M HCl medium. The color was stable for at least 2 hours. Effects of time, HCl concentration, gelatine and various ions have been studied. The system confirms the Beer's law over the concentration range investigated. Optimum condition range for measurement in 1.0 cm quartz cell is 0.1–0.5 μg/ml of zirconium. The molar absorptivity is 1.51×10⁵ liter mole^?1 cm^?1. The metal-ligand ratio of 1:4 was confirmed by Job's Continuous Variation method and the conditional stability constant of Zirconium-Arsenazo III Complex in 4 M HCl and 9 M HCl were also determined by this method.     

A new imidazolylquinoline for organic thin film transistor

A new active electronic material, 2-(naphtho[3,4]imidazol-2-yl)quinoline (NIQ), 1, has been synthesized and fully characterized. This compound exhibits field-effect carrier mobility and behaves as a p-type semiconductor (μ_FET = 0.148 cm²/V s at V_DS = 10 V). NIQ and its related imidazolylquinoline compounds may have possible applications as active materials in organic thin film transistors.     

Determination of mercury in human urine by neutron activation analysis,with lead diethyldithiocarbamate as a preconcentration agent

Lead diethyldithiocarbamate is an effective reagent for preconcentration of mercury in urine for neutron activation analysis. Sodium and bromine are removed from the sample by this procedure. As lead diethyldithiocarbamate is insensitive to neutron activation, radiochemical separation is not needed after neutron irradiation. Results from the analysis of urine collected from workers in caustic soda manufacturing plants are discussed.     

The effect of the steric hindrance on metallic cation conduction in polymer electrolytes

2,6-Di-t-butylphenol and oligo(ethylene oxide) bound covalently to polyisocyanate were synthesized and characterized. The ionic conductivities of their Li, Na, and K phenolates were studied at various temperatures. The conductivities were in the range of 10^?7?10^?5 S/cm at 30°C. The conductivity of Na and K salts was approximately 10² greater than that of the Li salts. The t-butyl groups serve to dissociate K and Na ions from the phenoxide. The cations, therefore, are more mobile as a result increasing the conductivity. The temperature dependence of ionic conductivity suggests that the migration of ions is controlled by segmental motion of the polymer, shown by linear curves obtained in Vogel–Tammann–Fulchere plots. The polyisocyanate backbone is a rather stiff structure, however, a flexible oligo(ethylene oxide) side chain forms complexes with metal ion. Since the ion transport is associated with the local movement of polymer segments, the rigidity of the polymer backbone does not have much influence on the ion mobility.     

Correspondence between the classical and quantum canonical transformation groups from an operator formulation of the wigner function

An explicit expression of the Wigner operator is derived, such that the Wigner function of a quantum state is equal to the expectation value of this operator with respect to the same state. This Wigner operator leads to a representation-independent procedure for establishing the correspondence between the inhomogeneous symplectic group applicable to linear canonical transformations in classical mechanics and the Weyl-metaplectic group governing the symmetry of unitary transformations in quantum mechanics.     

Experimental analysis of metal coated dielectric waveguides

This paper concerns the experimental characteristics of metal coated dielectric waveguides with a rectangular surface corrugation. Waveguide are designed to operate at a second Bragg frequency of 90 GHz. The period, height and the duty cycle of a rectangular grating were calculated using the chosen frequency. A metallic layer of aluminum is sputtered on one side of the slab waveguide. The purpose of the metallic layer is to simulate a layer of high density plasma on the surface of the waveguide similar to that obtained by optical excitation of semiconductor structures. Experiments were performed to examine the far field radiation pattern, attenuation constant and the dispersion relation. Due to the presence of the plasma layer there will be an angular shift in the far field radiation pattern. We have observed angular shifts of about 20 in the radiation pattern of the waveguide before and after coating. Measurements are made in the frequency range of 88–95 GHz. This waveguide structure can be used to design an electronically steerable antenna and an electronic phase shifter operating in the millimeter-wave frequency band.Supported in part by the Army Research Office.